Allows to modify
Thanks to this technology we are able to improve the productivity of a secondary metabolite, rebuild a metabolic pathway switching on or off particular genes (metabolic engineering), improve the characteristic of an enzyme, or insert in a host a complete cluster of your interest.
Polymerase chain reaction (PCR) is a key tool, that can be considered as the most powerful tool currently available in enzyme modification
Indeed, it is possible to generate a library of mutants that could be tested for the desired characteristics:
maximum yields, higher stability, low inhibition, high efficiency and low side activity.