Molecular biology is a branch of science that allows to modify a microorganism.
Thanks to this technology we are able to improve the productivity of a secondary metabolite, rebuild a metabolic pathway switching on or off particular genes (metabolic engineering), improve the characteristic of an enzyme, or insert in a host a complete cluster of your interest.
Polymerase chain reaction (PCR) is a key tool in this field and could be considered the most powerful instrument to use in enzyme modification.
Indeed, it is possible to generate a library of mutants that could be tested for the desired characteristics (maximum yields, higher stability, low inhibition, high efficiency and low side activity).
- Identification and optimization of gene sequences
- Homology modeling, docking and molecular dynamic
- Heterologous expression of proteins and peptides in bacteria and yeast
- Microbial genotyping
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